[發明專利]一種準確高效的單一試劑的低密度脂蛋白膽固醇檢測試劑

在審

• 申請號: 201810911785.6
• 申請日: 2018-08-10
• 公開（公告）號: CN109097436A
• 公開（公告）日: 2018-12-28
• 發明（設計）人: 羅維曉;胡曉飛;包興艷;王美麗
• 申請（專利權）人: 山東博科生物產業有限公司
• 主分類號: C12Q1/60
• 分類號: C12Q1/60;C12Q1/26;C12Q1/28;C12Q1/44
• 代理公司: 暫無信息
• 代理人: 暫無信息

1.一種準確高效的單一試劑的低密度脂蛋白膽固醇檢測試劑，所述試劑R的組成如下：

緩沖液_{················································}50mmol/L-100mmol/L，

氯化鎂_{················································}2.5g/L-5g/L，

多聚陰離子_{············································}3g/L-5g/L，

糖類環狀物_{············································}1g/L-5g/L，

HDAOS（N-(2-羥基-3-磺丙基)-3,5-二甲基氧基苯胺鈉鹽）_·····0.1g/L-1g/L，

表面活性劑1_{···········································}0.1g/L-1g/L，

表面活性劑2_{···········································}0.1g/L-1g/L

表面活性劑3_{···········································}5g/L-10g/L

抗壞血酸氧化酶_{········································}1KU/L-5KU/L，

膽紅素氧化酶_{··········································}1KU/L-3KU/L，

膽固醇脂酶_{············································}1KU/L-3KU/L，

膽固醇氧化酶_{··········································}0.5KU/L-3KU/L，

過氧化物酶_{············································}1KU/L-3KU/L，

4-AA_{··················································}0.1g/L-0.3g/L，

防腐劑_{················································}1mL/L-3mL/L。

2.根據權利要求1所述的一種準確高效的單一試劑的低密度脂蛋白膽固醇檢測試劑，試劑R中緩沖液為25℃，PH=6.5的MES緩沖液。

3.根據權利要求1所述的一種準確高效的單一試劑的低密度脂蛋白膽固醇檢測試劑，所述多聚陰離子為硫酸葡聚糖5萬（山海西寶生物）。

4.根據權利要求1所述的一種準確高效的單一試劑的低密度脂蛋白膽固醇檢測試劑，所述糖類環狀物為β-環糊精。

5.根據權利要求1所述的一種準確高效的單一試劑的低密度脂蛋白膽固醇檢測試劑，所述表面活性劑1、表面活性劑2、表面活性劑3分別為AMPHITOL 20AB、壬基酚聚氧乙烯月桂醚 NP-07、聚氧乙烯聚丙烯醚嵌段共聚物（plUronic F-68）。

6.根據權利要求1所述的一種準確高效的單一試劑的低密度脂蛋白膽固醇檢測試劑，所述防腐劑為PC-300。

7.根據權利要求1-6所述的一種準確高效的單一試劑的低密度脂蛋白膽固醇檢測試劑為單一檢測試劑。

8.根據權利要求1-7所述的一種準確高效的單一試劑的低密度脂蛋白膽固醇檢測試劑，來檢測低密度脂蛋白膽固醇的檢測方法，使用全自動生化分析儀利用終點法進行測定，檢測主波長為546nm，副波長為700nm。