[發明專利]一種穩定、抗干擾能力強的血清磷脂檢測試劑及檢測方法有效
| 申請號： | 201510971392.0 | 申請日： | 2015-12-22 |
| 公開（公告）號： | CN105543336B | 公開（公告）日： | 2019-03-12 |
| 發明（設計）人： | 李志明;甘宜梧;趙新;李靜 | 申請（專利權）人： | 山東博科生物產業有限公司 |
| 主分類號： | C12Q1/44 | 分類號： | C12Q1/44;C12Q1/28;C12Q1/26 |
| 代理公司： | 暫無信息 | 代理人： | 暫無信息 |
| 地址： | 250200 山東省濟南*** | 國省代碼： | 山東;37 |
| 權利要求書： | 查看更多 | 說明書： | 查看更多 |
| 摘要： | |||
| 搜索關鍵詞： | 一種 穩定 抗干擾 能力強 血清 磷脂 檢測 試劑 方法 | ||
1.一種血清磷脂檢測試劑，其特征在于包括試劑R1和試劑R2，所述試劑R1和試劑R2的組成如下：
試劑R1的組分為：
緩沖液··········································.........··100mmol/L，
磷脂酶D·················································1KU/L，
N-乙基-N-(2-羥基-3-磺丙基)-3'5-二甲氧基苯胺鈉鹽·········1.4mmol/L，
抗壞血酸氧化酶··········································2KU/L，
膽紅素氧化酶············································2KU/L，
聚乙二醇6000············································10g/L，
蔗糖····················································20g/L，
黃原膠··················································0.5g/L，
甘露醇··················································20g/L，
海藻糖··················································10g/L，
牛血清白蛋白············································2g/L，
聚氧丙烯氧化乙烯甘油醚··································1g/L，
防腐劑··················································0.5g/L；
2）試劑R2的組分為：
緩沖液··················································100mmol/L，
4-氨基氨替吡啉··········································2.5mmol/L，
過氧化物酶··············································20KU/L，
膽堿氧化酶··············································8KU/L，
聚乙二醇6000············································10g/L，
蔗糖····················································20g/L，
黃原膠··················································0.5g/L，
甘露醇··················································20g/L，
海藻糖··················································10g/L，
牛血清白蛋白············································2g/L，
聚氧丙烯氧化乙烯甘油醚··································1g/L，
防腐劑··················································0.5g/L；
所述試劑R1中的緩沖液和試劑R2中的緩沖液均為25℃，pH為7.2的HEPES緩沖液；
所述試劑R1中的防腐劑和試劑R2中的防腐劑均為NaN3；
所述試劑R1與試劑R2的比例為4：1。
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