[發明專利]用于轉基因植物中生產類胡蘿卜素的相關基因的合成

無效

• 申請號: 01105878.1
• 申請日: 2001-04-06
• 公開（公告）號: CN1380415A
• 公開（公告）日: 2002-11-20
• 發明（設計）人: 姚泉洪;彭日荷;熊愛生
• 申請（專利權）人: 上海永業農科生物工程有限公司;上海市農業科學院生物技術研究中心
• 主分類號: C12N15/52
• 分類號: C12N15/52;C12N15/63;C12N15/70;C12P23/00;C07C403/24;A01H1/00
• 代理公司: 上海智信專利代理有限公司
• 代理人: 費開逵

1、由來自副球菌的垅牛兒基垅牛兒基焦磷酸合成酶基因(crtE)；八氫番茄紅素合成酶基因(crtB)；八氫番茄紅素去飽和酶基因(crtI)；番茄紅素β環化酶基因(crtY)；β-胡蘿卜素羥化酶基因(crtZ)和β-胡蘿卜素酮酶基因(crtW)，其特征在于由前述六種基因改造成主導蝦青素生物合成的crtW?crtY基因和crtI?crtZ及crtB?crtE聯合基因。

2、根據權利要求1所述主導蝦青素生物合成的crtWcrtY基因核苷酸和氨基酸序列如下：

ATGTCCGCACACGCACTCCCAAAAGCAGACCTCACCGCAACCTCCCTCATCGTCTCCGGTGGTATCATCGCA--?72

?M??S??A??H??A??L??P??K??A??D??L??T??A??T??S??L??I??V??S??G??G??I??I??A

GCATGGCTCGCACTCCACGTCCACGCACTCTGGTTCCTCGACGCAGCAGCACACCCAATCCTCGCAGTCGCA--144

?A??W??L??A??L??H??V??H??A??L??W??F??L??D??A??A??A??H??P??I??L??A??V??A

AACTTCCTCGGTCTCACCTGGCTCTCCGTCGGTCTCTTCATCATCGCACACGACGCAATGCACGGTTCCGTC--216

?N??F??L??G??L??T??W??L??S??V??G??L??F??I??I??A??H??D??A??M??H??G??S??V

GTCCCAGGTAGACCAAGAGCAAACGCAGCAATGGGTCAACTCGTCCTCTGGCTCTACGCAGGTTTCTCCTGG--288

?V??P??G??R??P??R??A??N??A??A??M??G??Q??L??V??L??W??L??Y??A??G??F??S??W

AGAAAGATGATCGTCAAGCACATGGCACACCACAGACACGCAGGTACTGACGACGACCCAGACTTCGACCAC--360

?R??K??M??I??V??K??H??M??A??H??H??R??H??A??G??T??D??D??D??P??D??F??D??H

GGTGGTCCAGTCAGATGGTACGCAAGATTCATCGGTACTTACTTCGGTTGGAGAGAAGGTCTCCTCCTCCCA--432

?G??G??P??V??R??W??Y??A??R??F??I??G??T??Y??F??G??W??R??E??G??L??L??L??P

GTCATCGTCACCGTCTACGCACTCATCCTCGGTGACAGATGGATGTACGTCGTCTTCTGGCCACTCCCATCC--504

?V??I??V??T??V??Y??A??L??I??L??G??D??R??W??M??Y??V??V??F??W??P??L??P??S

ATCCTCGCATCCATCCAACTCTTCGTCTTCGGCACCTGGCTCCCACACAGACCAGGTCACGACGCATTCCCA--576

?I??L??A??S??I??Q??L??F??V??F??G??T??W??L??P??H??R??P??G??H??D??A??F??P

GACAGACACAACGCAAGATCCTCCAGAATCTCCGACCCAGTCTCCCTCCTCACCTGCTTCCACTTCGGTGGC--648

?D??R??H??N??A??R??S??S??R??I??S??D??P??V??S??L??L??T??C??F??H??F??G??G

TACCACCACGAACACCACCTCCACCCAACCGTCCCTTGGTGGAGACTCCCATCCACCAGAACCAAAGGCGAC--720

?Y??H??H??E??H??H??L??H??P??T??V??P??W??W??R??L??P??S??T??R??T??K??G??D

ACCGCACAACTCCTCAACTTCGACCTCCTCAAACTCGCAGGCGACGTCGAATCCAACCCAGGCCCAATGACC--792

?T??A??Q??L??L??N??F??D??L??L??K??L??A??G??D??V??E??S??N??P??G??P??M??T

CACGACGTCCTCCTCGCAGGTGCAGGTCTCGCAAACGGTCTCATCGCACTCGCACTCAGAGCAGCAAGACCA--864

?H??D??V??L??L??A??G??A??G??L??A??N??G??L??I??A??L??A??L??R??A??A??R??P

GACCTCAGAGTCCTCCTCCTCGACCACGCAGCAGGTCCATCCGAAGGTCACACCTGGTCCTGCCACGACCCA--936

?D??L??R??V??L??L??L??D??H??A??A??G??P??S??E??G??H??T??W??S??C??H??D??P

GACCTCTCCCCACACTGGCTCGCAAGACTCAAGCCACTCAGAAGAGCAAACTGGCCAGACCAAGAAGTCAGA-1008

?D??L??S??P??H??W??L??A??R??L??K??P??L??R??R??A??N??W??P??D??Q??E??V??R

TTCCCAAGACACGCAAGAAGACTCGCAACCGGCTACGGCTCCCTCGACGGTGCAGCACTCGCAGACGCAGTC-1080

?F??P??R??H??A??R??R??L??A??T??G??Y??G??S??L??D??G??A??A??L??A??D??A??V

GTCAGATCCGGTGCAGAAATCAGATGGGACTCCGACATCGCACTCCTCGACGCACAAGGTGCAACCCTCTCC-1152

?V??R??S??G??A??E??I??R??W??D??S??D??I??A??L??L??D??A??Q??G??A??T??L??S

TGCGGTACTAGAATCGAAGCAGGCTCCGTCCTCGACGGCAGAGGCCCACACCCATCCAGACACCTCACCCTC-1224

?C??G??T??R??I??E??A??G??S??V??L??D??G??R??G??P??H??P??S??R??H??L??T??L

GGCTTCCACAAGTTCCTCGGTGTCGAAATCGAAACCGACAGACCACACGGTGTCCCAAGACCAGTCATCATG-1296

?G??F??H??K??F??L??G??V??E??I??E??T??D??R??P??H??G??V??P??R??P??V??I??M

GACGGCACCGTCACCCAAAGAGACGGCTACGGCTTCATCTACCTCCTCCCATTCTCCAGAACCAGAATCCTC-1368

?D??G??T??V??T??Q??R??D??G??Y??G??F??I??Y??L??L??P??F??S??R??T??R??I??L

ATCGAAGACACCAGATACTCCGACGGCGGCGACCTCGACGACGACGCACTCGCAGCAGCATCCCAAGACTAC-1440

?I??E??D??T??R??Y??S??D??G??G??D??L??D??D??D??A??L??A??A??A??S??Q??D??Y

GCAAGACAACAAGGCTGGACCGGTGCAGAAGTCAGAAGAGAAAGAGGCATCCTCCCAATCGCACTCGCACAC-1512

?A??R??Q??Q??G??W??T??G??A??E??V??R??R??E??R??G??I??L??P??I??A??L??A??H

GACGCAGCAGGTTTCTGGGCAGACCACGCAGAAGGTCCAGTCCCAGTCGGTCTCAGAGCAGGCTTCTTCCAC-1584

?D??A??A??G??F??W??A??D??H??A??E??G??P??V??P??V??G??L??R??A??G??F??F??H

CCAGTCACCGGCTACTCCCTCCCATACGCAGCACAAGTCGCAGACGTCGTCGCAGGTCTCTCCGGTCCACCA-1556

?P??V??T??G??Y??S??L??P??Y??A??A??Q??V??A??D??V??V??A??G??L??S??G??P??P

GGCACCGACGCACTCAGAGGTGCAATCAGAGACTACGCAATCGACAGAGCAAGAAGAGACAGATTCCTCAGA-1728

?G??T??D??A??L??R??G??A??I??R??D??Y??A??I??D??R??A??R??R??D??R??F??L??R

CTCCTCAACAGAATGCTCTTCAGAGGCTGCGCACCAGACAGAAGATACACCCTCCTCCAAAGACTCTACAGA-1800

?L??L??N??R??M??L??F??R??G??C??A??P??D??R??R??Y??T??L??L??Q??R??L??Y??R

ATGCCACACGGTCTCATCGAAAGATTCTACGCAGGTAGACTCTCCGTCGCAGACCAACTCAGAATCGTCACC-1872

?M??P??H??G??L??I??E??R??F??Y??A??G??R??L??S??V??A??D??Q??L??R??I??V??T

GGCAAGCCACCAATCCCACTCGGCACCGCAATCAGATGCCTCCCAGAAAGACCACTCCTCAAGGAAAACGCA-1994

?G??K??P??P??I??P??L??G??T??A??I??R??C??L??P??E??R??P??L??L??K??E??N??A