[發(fā)明專利]水稻滲透脅迫應(yīng)答蛋白OsU496A在提高水稻抗?jié)B透脅迫能力方面的應(yīng)用

在審

• 申請(qǐng)?zhí)枺?/td>: 202011060584.3
• 申請(qǐng)日: 2020-09-30
• 公開（公告）號(hào): CN112142834A
• 公開（公告）日: 2020-12-29
• 發(fā)明（設(shè)計(jì)）人: 呂冰;王家豪;方是珍;王藝霖;陳洪晴;陳開;楊超;陳云
• 申請(qǐng)（專利權(quán)）人: 揚(yáng)州大學(xué)
• 主分類號(hào): C07K14/415
• 分類號(hào): C07K14/415;C12N15/29;C12N15/82;A01H5/00;A01H6/46
• 代理公司: 揚(yáng)州蘇中專利事務(wù)所(普通合伙) 32222
• 代理人: 沈志海

1.水稻滲透脅迫應(yīng)答蛋白OsU496A在提高水稻抗?jié)B透脅迫能力方面的應(yīng)用，其特征是：首先將含正反RNAi水稻滲透脅迫應(yīng)答蛋白OsU496A編碼基因特異性片段的干擾表達(dá)載體導(dǎo)入水稻細(xì)胞、組織或器官中，降低水稻細(xì)胞、組織或器官中水稻滲透脅迫應(yīng)答蛋白OsU496A編碼基因的表達(dá)量，然后將水稻細(xì)胞、組織或器官培養(yǎng)成植株，使水稻滲透脅迫應(yīng)答蛋白OsU496A編碼基因的表達(dá)量降低，得到在滲透脅迫下具有良好生理指標(biāo)、抗?jié)B透脅迫能力提升的水稻。

2.根據(jù)權(quán)利要求1所述的應(yīng)用，其特征是：水稻滲透脅迫應(yīng)答蛋白OsU496A來源于OryzasativaJaponicaGroup；具有下列氨基酸殘基序列之一：

(1)SEQIDNo:1；

(2)將SEQIDNo:1的氨基酸殘基序列經(jīng)過編輯且對(duì)植物的生長(zhǎng)發(fā)育具有調(diào)控作用的蛋白質(zhì)；

SEQIDNo:1由388個(gè)氨基酸殘基組成；

SEQ ID No:1為：

Met Gly Asn Ser Ser Ser Ser Gly Ser His Arg Pro Pro Arg Pro Ala

Ser Ser Glu Ser Ala Leu Pro Pro Ala Ala Ala Ala Ala Glu Glu Leu

Ser Ser Tyr Glu Ala Ala Cys Arg Ser Asp Pro Glu Leu Arg Thr Phe

Asp Thr Thr Leu Gln Arg Arg Thr Ser Arg Ala Ile Ser Thr Leu Ala

Val Gly Val Glu Val Arg Ser Leu Ser Leu Glu Ser Leu Arg Glu Val

Thr Gly Cys Leu Leu Asp Met Asn Gln Glu Val Val Arg Val Ile Leu

Asp Cys Lys Lys Asp Ile Trp Lys Ser Pro Glu Leu Phe Asp Leu Val

Glu Asp Tyr Phe Glu Ser Ser Leu His Thr Leu Asp Phe Cys Thr Ala

Leu Asp Lys Cys Leu Lys Arg Ala Arg Asp Ser Gln Leu Leu Leu His

Val Ala Leu Gln Arg Phe Asp Asp Glu Glu Asp Asn Asp Ala Ala Ala

Ala Gly Gln Glu Asp Ala Ala Pro Ser Ala Arg Tyr Ala Arg Thr Leu

His Glu Leu Arg Gln Phe Lys Ala Ala Gly Asp Pro PheThr Glu Glu

Phe Phe Ser Ala Phe Gln Ala Val Tyr Arg Gln Gln Leu Thr Met Leu

Glu Lys Leu Gln Gln Arg Lys His Arg Leu Asp Lys Lys Val Arg Ala

Ile Lys Ala Trp Arg Arg Val Ser Ser Ile Ile Phe Ala Thr Thr Phe

Ala Ala Val Leu Ile Cys Ser Val Val Ala Ala Ala Ile Ala Ala Pro

Pro Val Ala Ala Ala Leu Ala Ala Ala Ala Ser Ile Pro Val Gly Ser

Met Gly Lys Trp Ile Asp Ser Leu Leu Lys Gly Tyr Gln Asp Ala Leu

Arg Gly Gln Lys Glu Val Val Ser Ala Met Gln Val Gly Thr Phe Ile

Ala Ile Lys Asp Leu Asp Ser Ile Arg Val Leu Ile Asn Arg Val Glu

Leu Glu Ile Ser Ser Met Ile Asp Cys Val Glu Phe Ala Glu Arg Asp

Glu Glu Ala Val Lys Phe Gly Val Glu Glu Ile Lys Lys Lys Leu Glu

Val Phe Met Lys Ser Val Glu Asp Leu Gly Glu Gln Ala Asp Arg Cys

Ser Arg Asp Ile Arg Arg Ala Arg Thr Val Val Leu Gln Arg Ile Ile

Arg His Pro Ser

。