[發明專利]一種與皮膚老化表征相關的易感基因檢測panel及其應用

有效

• 申請號: 201910515358.0
• 申請日: 2019-06-14
• 公開（公告）號: CN110904207B
• 公開（公告）日: 2022-07-29
• 發明（設計）人: 張煜婷;聶蘇秦;俞瑞卿;張穎
• 申請（專利權）人: 陜西九州醫學檢驗有限公司
• 主分類號: C12Q1/6883
• 分類號: C12Q1/6883;C12N15/11
• 代理公司: 北京匯信合知識產權代理有限公司 11335
• 代理人: 吳甘棠

1.一種與皮膚老化表征相關的易感基因檢測panel，其特征在于，所述檢測panel用于檢測人基因組DNA的皮膚老化表征相關易感基因，所述檢測panel包括45個SNP位點的多重PCR擴增引物和45個SNP位點的單堿基延伸引物；

所述45個SNP位點為rs1800012、rs200519781、rs4880、rs1001179、rs10733310、rs11084111、rs2031526、rs1800795、rs185146、rs1800566、rs1805007、rs1060573、rs1426654、rs322458、rs3918242、rs1042602、rs873601、rs11084109、rs2066853、rs642742、rs1799750、rs17833789、rs12913832、rs679620、rs4268748、rs251468、rs885479、rs2228479、rs12203592、rs2071307、rs146466242、rs1015362、rs1695、rs4911414、rs10810635、rs747650、rs74653330、rs6059655、rs2303067、rs3126085、rs62543565、rs2228478、rs591058、rs1800255、rs2011616；

所述45個SNP位點的多重PCR擴增引物的序列為SEQ ID NO.1和SEQ ID NO.2、SEQ IDNO.3和SEQ ID NO.4、SEQ ID NO.5和SEQ ID NO.6、SEQ ID NO.7和SEQ ID NO.8、SEQ IDNO.9和SEQ ID NO.10、SEQ ID NO.11和SEQ ID NO.12、SEQ ID NO.13和SEQ ID NO.14、SEQID NO.15和SEQ ID NO.16、SEQ ID NO.17和SEQ ID NO.18、SEQ ID NO.19和SEQ ID NO.20、SEQ ID NO.21和SEQ ID NO.22、SEQ ID NO.23和SEQ ID NO.24、SEQ ID NO.25和SEQ IDNO.26、SEQ ID NO.27和SEQ ID NO.28、SEQ ID NO.29和SEQ ID NO.30、SEQ ID NO.31和SEQID NO.32、SEQ ID NO.33和SEQ ID NO.34、SEQ ID NO.35和SEQ ID NO.36、SEQ ID NO.37和SEQ ID NO.38、SEQ ID NO.39和SEQ ID NO.40、SEQ ID NO.41和SEQ ID NO.42、SEQ IDNO.43和SEQ ID NO.44、SEQ ID NO.45和SEQ ID NO.46、SEQ ID NO.47和SEQ ID NO.48、SEQID NO.49和SEQ ID NO.50、SEQ ID NO.51和SEQ ID NO.52、SEQ ID NO.53和SEQ ID NO.54、SEQ ID NO.55和SEQ ID NO.56、SEQ ID NO.57和SEQ ID NO.58、SEQ ID NO.59和SEQ IDNO.60、SEQ ID NO.61和SEQ ID NO.62、SEQ ID NO.63和SEQ ID NO.64、SEQ ID NO.65和SEQID NO.66、SEQ ID NO.67和SEQ ID NO.68、SEQ ID NO.69和SEQ ID NO.70、SEQ ID NO.71和SEQ ID NO.72、SEQ ID NO.73和SEQ ID NO.74、SEQ ID NO.75和SEQ ID NO.76、SEQ IDNO.77和SEQ ID NO.78、SEQ ID NO.79和SEQ ID NO.80、SEQ ID NO.81和SEQ ID NO.82、SEQID NO.83和SEQ ID NO.84、SEQ ID NO.85和SEQ ID NO.86、SEQ ID NO.87和SEQ ID NO.88、SEQ ID NO.89和SEQ ID NO.90所示的核苷酸序列；

所述45個SNP位點的單堿基延伸引物的序列為SEQ ID NO.91、SEQ ID NO.92、SEQ IDNO.93、SEQ ID NO.94、SEQ ID NO.95、SEQ ID NO.96、SEQ ID NO.97、SEQ ID NO.98、SEQ IDNO.99、SEQ ID NO.100、SEQ ID NO.101、SEQ ID NO.102、SEQ ID NO.103、SEQ ID NO.104、SEQ ID NO.105、SEQ ID NO.106、SEQ ID NO.107、SEQ ID NO.108、SEQ ID NO.109、SEQ IDNO.110、SEQ ID NO.111、SEQ ID NO.112、SEQ ID NO.113、SEQ ID NO.114、SEQ ID NO.115、SEQ ID NO.116、SEQ ID NO.117、SEQ ID NO.118、SEQ ID NO.119、SEQ ID NO.120、SEQ IDNO.121、SEQ ID NO.122、SEQ ID NO.123、SEQ ID NO.124、SEQ ID NO.125、SEQ ID NO.126、SEQ ID NO.127、SEQ ID NO.128、SEQ ID NO.129、SEQ ID NO.130、SEQ ID NO.131、SEQ IDNO.132、SEQ ID NO.133、SEQ ID NO.134和SEQ ID NO.135所示的核苷酸序列。