[發明專利]脯氨酸羥化酶及其應用

有效

• 申請號: 201610971207.2
• 申請日: 2016-11-04
• 公開（公告）號: CN106834244B
• 公開（公告）日: 2021-07-06
• 發明（設計）人: 洪浩;詹姆斯·蓋吉;盧江平;張娜;于文燕;劉芳;李艷君;黃鑫;高娟;張克儉;馬玉磊;魏俊璐
• 申請（專利權）人: 凱萊英醫藥集團(天津)股份有限公司;凱萊英生命科學技術(天津)有限公司;天津凱萊英制藥有限公司;凱萊英醫藥化學（阜新）技術有限公司;吉林凱萊英醫藥化學有限公司
• 主分類號: C12N9/02
• 分類號: C12N9/02;C12N15/53;C12P13/24
• 代理公司: 北京康信知識產權代理有限責任公司 11240
• 代理人: 趙囡囡;吳貴明

1.一種脯氨酸羥化酶，其特征在于，為

SEQ ID NO:2所示的氨基酸序列經過氨基酸的突變且具有脯氨酸羥化酶活性的蛋白質，所述突變發生在I39位點且所述突變為I39K或I39R。

2.根據權利要求1所述的脯氨酸羥化酶，其特征在于，所述突變為如下任意一種組合：E27K+I39K、I39K+K123Q、I39K+K123D、I39R+K123D、E27K+I39K+K123D、E27K+I39R、I39R+N165H、I39K+N165H、E27K+I39R+K123D、S37C+I39K+I223V+K123D、E27K+S37C+I39K+K123D+I98V、E27K+S37C+I39K+K123D+I223V、F26L+E27K+I39K+K123D、I223V+S37C+E27K+I39K、I39R+Y35W、E27K+I39K+Y35W、E27K+S37C+I39K+I98V+K123D+I223V、E27K+I39K+K123D+N165H、E27K+I39R+Y35W、S37C+I39K+N165H、E27K+I39K+D30S、E27K+I39K+E34N、E27K+I39K+E34G、E27K+I39K+S37W、E27K+I39K+S37E、E27K+I39K+Y35K、E27K+I39K+S37N、E27K+I39K+K123Q、E27K+I39K+K123S、E27K+I39K+K106Q+K112E、I98V+E27K+I39K、E27K+I39K+I118F、E27K+I39K+S37T、E27K+I39K+K123I、E27K+I39K+E34L、E27K+I39K+S37F+I58T、E27K+I39K+S37F+I58Y、E27K+I39K+S37F+I58A、E27K+I39K+S37F+I58R、E27K+I39K+S37F+I58V、E27K+I39K+S37F+I58S、E27K+I39K+S37N+I58C、E27K+D30S+I39K+I58R+K112E、E27K+E34N+I39K+I58Y+I223V、E27K+S37N+I39K+I58Y+D173G、E27K+S37F+I39K+I58Y+D173G、E27K+I39K+S37N+I58A、E27K+I39K+S37N+I58R+K123Q、E27K+S37F+I39K+I58Y+D173G+I118R、E27K+E34L+S37N+I39K+I58R、E27K+E34L+S37N+I39K+I58Y+D173G、E27K+E34L+S37N+I39K+I58Y+D173G+K123Q、H14R+E27K+D30S+E34G+I39K+I98V+K106Q+K112E+I223V、H14R+E27K+E34N+I39K+I98V+K106Q+K112E+I223V、H14R+E27K+E34G+I39K+I98V+K106Q+K112E+I223V、H14R+E27K+E34G+S37W+I39K+I98V+K106Q+K112E+I223V、H14R+E27K+E34G+S37F+I39K+I98V+K106Q+K112E+I223V、H14R+E27K+E34G+S37E+I39K+I98V+K106Q+K112E+I223V、H14R+E27K+E34G+Y35K+I39K+I98V+K106Q+K112E+I223V、H14R+E27K+E34G+S37N+I39K+I98V+K106Q+K112E+I223V、H14R+E27K+E34G+I39K+I98V+K106Q+K112E+K123Q+I223V、H14R+E27K+E34G+I39K+I98V+K106Q+K112E+K123S+I223V、H14R+E27K+E34G+I39K+I58A+I98V+K106Q+K112E+I223V、H14R+E27K+E34G+I39K+I98V+K106Q+K112E+I118F+I223V、H14R+E27K+E34G+S37T+I39K+I98V+K106Q+K112E+I223V、H14R+E27K+E34G+I39K+I58V+I98V+K106Q+K112E+I223V、H14R+E27K+E34G+I39K+I98V+K106Q+K112E+K123I+I223V。

3.一種DNA分子，其特征在于，所述DNA分子編碼權利要求1或2所述的脯氨酸羥化酶。