[發明專利]一種阿特拉津降解菌及其應用

在審

• 申請號: 201410839729.8
• 申請日: 2014-12-30
• 公開（公告）號: CN104762227A
• 公開（公告）日: 2015-07-08
• 發明（設計）人: 巴扎諾夫·德米特里;李紅梅;李成云;李紀順;楊合同
• 申請（專利權）人: 山東省科學院中日友好生物技術研究中心
• 主分類號: C12N1/20
• 分類號: C12N1/20;B09C1/10;C02F3/34;C12R1/06;C02F101/38
• 代理公司: 濟南舜源專利事務所有限公司 37205
• 代理人: 于曉曉

技術領域

本發明涉及菌株及其應用技術領域，特別是涉及一種阿特拉津降解產脲節桿菌及其應用。

背景技術

阿特拉津【2-氯-4-(乙胺)-6-(異丙胺)-1,3,5阿特拉津】是主要的s-三嗪類除草劑成員，三嗪類除草劑主要包括西瑪津、氰草津、撲滅津、特丁津、撲草凈、特丁凈、莠滅凈和西瑪通。1958年，阿特拉津在美國和瑞士注冊并開始應用(Heri?W,Pfister?F,Carroll?B,Parshley?T,Nabors?JB(2008)Production,developmnent,and?registration?of?triazine?herbicides.In:LeBaron?HM,McFarland?J,Burnside?OC,editors.The?triazine?herbicides:50?years?revolutionizing?agriculture.San?Diego:Elsevier.pp.31-43.)。因其在玉米種植中顯著的除草效果，阿特拉津迅速成為最暢銷和應用最廣泛的除草劑之一(Thelin?GP,Stone?WW(2013)Estimation?of?annual?agricultural?pesticide?use?for?counties?of?the?conterminous?United?States,1992–2009.U.S.Geological?Survey,Reston,Virginia:2013.54?p.Available:http://pubs.usgs.gov/sir/2013/5009/pdf/sir20135009.pdf)，但是，檢測到其對土壤、地下水、地表水以及盆地有普遍污染(Battaglin?WA,Rice?KC,Focazio?MJ,Salmons?S,Barry?RX(2009)The?occurrence?of?glyphosate,atrazine,and?other?pesticides?in??vernal?pools?and?adjacent?streams?in?Washington,DC,Maryland,Iowa,and?Wyoming,2005–2006.Environ?Monit?Assess?155:281-307；Kookana?RS,Baskaran?S,Naidu?R(1998)Pesticide?fate?and?behaviour?in?Australian?soils?in?relation?to?contamination?and?management?of?soil?and?water:a?review.Australian?Journal?of?Soil?Research?36:715-764；S,Budimir?M,D,Ne?N(2002)Residues?of?atrazine?in?agricultural?areas?of?Serbia.Journal?of?Serbian?Chemical?Society?67:887-892；Pick?FE,van?Dick?LP,Botha?E(1992)Atrazine?in?ground?and?surface?water?in?maize?production?areas?of?the?Transvaal,South?Africa.Chemosphere?25:335-341；Shomar?BH,Müller?G,Yahya?A(2006)Occurrence?of?pesticides?in?groundwater?and?topsoil?of?the?Gaza?Strip.Water?Air?Soil?Pollut?171:237–251；Aslam?M,Alam?M,Rais?S(2013)Detection?of?atrazine?and?simazine?in?ground?water?of?Delhi?using?high?performance?liquid?chromatography?with?ultraviolet?detector.Current?World?Environment?8:323-329.)。在北京和上海地區的地下水和水庫中檢測到阿特拉津及其脫烷基化衍生物(Ye?C,Gong?A,Wang?X,Zheng?H,Lei?Z(2001)Distribution?of?atrazine?in?a?crop-soil-groundwater?system?at?Baiyangdian?lake?area?in?China.J?Environ?Sci(China)13:148-152；Ren?J,Jiang?K(2002)Atrazine?and?its?degradation?products?in?surface?and?ground?waters?in?Zhangjiakou?district,China.Chin?Sci?Bull47:1612-1615；塔娜,馮建芳,孫成,周芳(2005).太湖梅粱灣水體中阿特拉津的毛細管氣相色譜法測定.環境污染與防治.27(8):634-636；馬曉雁,高乃云,李青松,徐斌,樂林生,吳今明?(2006).固相萃取高效液相色譜檢測原水中微量內分泌干擾物.給水排水.32(1):6-10.)，表明中國有大面積的土壤和水源遭到污染。