[發(fā)明專利]利用顏色和外來蜂群提取物刺激提高胡蜂毒采集量的方法

有效

• 申請?zhí)枺?/td>: 201310624815.2
• 申請日: 2013-11-28
• 公開（公告）號: CN103636528A
• 公開（公告）日: 2014-03-19
• 發(fā)明（設計）人: 焦春香;劉衡;趙海榮;巫秀美;趙昱
• 申請（專利權(quán)）人: 大理學院
• 主分類號: A01K59/00
• 分類號: A01K59/00
• 代理公司: 暫無信息
• 代理人: 暫無信息

技術(shù)領域

本發(fā)明涉及生物醫(yī)藥技術(shù)領域，具體而言，本發(fā)明涉及一種利用昆蟲生理特性提高胡蜂毒素采集量的技術(shù)。其關鍵技術(shù)特征在于將外框涂成紅色的取毒器上涂上外來蜂群的蜂巢、蜂體、蜂排泄物和/或蜂毒中得到的提取物的揮發(fā)性溶液，再以紅色襯墊做底色對胡蜂進行雙重刺激，增大蜂群的排毒量和攻擊次數(shù)。本法的蜂毒采集量比使用常規(guī)采毒器的蜂毒采集量提高了23～35%。本方法適用于對長腹胡蜂科、鈴腹胡蜂科、異腹胡蜂科、狹腹胡蜂科、馬蜂科、胡蜂科等胡蜂總科的經(jīng)濟昆蟲進行生物毒素采集，滿足市場的短缺和采毒創(chuàng)新技術(shù)的不足，增加貧困山區(qū)經(jīng)濟昆蟲養(yǎng)殖戶收入，具有顯著的經(jīng)濟價值和社會效益。

背景技術(shù)

蜂毒是具有高度生物學活性和藥理學活性的復雜混合物。胡蜂毒不僅具有過敏性，且可以造成血纖維蛋白溶解作用、對骨骼肌的破壞、神經(jīng)系統(tǒng)癥狀和視覺障礙引發(fā)的過敏性休克等（Lombardini,C等,Am?J?Emerg?Med,2009,153:131-135；Patriarca,G等,Intl?J?Immunopathol?Pharmacol,2008,21:669-677；Schultze-Werninghaus,C等,PediatrAllergy?Immunol,1999,10:133-137；Puchalska-Niedbal,L等,Ann?Acad?Med?Stetin,2007,53Suppl1:90-93；Rocha,T等,Toxicon,2007,50:589-599）；胡蜂毒還顯示對心血管、降壓、抗菌、抑制乙酰膽堿酯酶等許多有前景的生理活性（Rubin,Y等,Cardiovascular?haemodynamics?of?oriental?hornet?venom?sac?extract,Pharmacol?Toxicol,1993,72:268-272；Ho,CL等，Structures?and?biological?activities?of?new?wasp?venom?peptides?isolated?feom?the?black-bellied?hornet(Vespa?basalis)venom.Toxicon,1998,36:609-617；Konno,K等,Molecular?determinants?of?binding?of?a?wasp?toxin(PMTXs)and?its?analogs?in?the?Na+channels?proteins.Neurosci?Lett,2000,285:29-32；Ho,CL等,Enhancing?the?hypotensive?effect?and?diminishing?the?cytolytic?activity?of?hornet?mastoparan?B?by?D-amino?acid?substitution,Toxicon,2001,39:1561-1566；Dami,MP等,Antibacterial?and?proteolytic?activity?in?venom?from?the?endoparasitic?wasp?Pimpla?hypochondriaca(Hymenoptera:Ichneumonidae),J?Insect?Physiol,2003,49:945-954；Wang,JL等,Isolated?prolongation?of?activated?partial?thromboplastin?time?following?wasp?sting,Acta?Paediatr?Taiwan,2005,46:164-165；Abt,M等,Characterization?of?phenoloxidase?activity?in?venom?from?the?ectoparasitoid?Nasonia?vitripennis(Walker)(Hymenoptera:Pteromalidae),J?Invertebr?Pathol,2007,94:108-118；Polsinelli,GA等,Insect-specific?irreversible?inhibitors?of?acetylcholineesterase?in?pests?including?the?bed?bug,the?eastern?yellowjacket,German?and?American?cockroaches,and?the?confused?flour?beetle,Chem?Biol?Interact,2010,187:142-147；Ye,J等,A?defensin?antimicrobial?peptide?from?the?venoms?of?Nasonia?vitripennis,Toxicon,2010,56:101-106）。