[發明專利]運用比較基因組學快速鑒定薺菜抗白粉病基因

在審

• 申請號: 201310541423.X
• 申請日: 2013-11-04
• 公開（公告）號: CN104611409A
• 公開（公告）日: 2015-05-13
• 發明（設計）人: 錢孝英;袁偉;錢春桃
• 申請（專利權）人: 常熟市董浜鎮北港蔬菜專業合作社
• 主分類號: C12Q1/68
• 分類號: C12Q1/68;G06F19/24;C12N15/29;C07K14/415
• 代理公司: 無
• 代理人: 無

1.薺菜抗白粉病基因，其特征在于選自下列4個基因或其之一：

carubv10022893m.g

氨基酸序列：

MAIKERTLEETPTWAVAVVCFVLLFISIMIEYFLHFVGHWFKKKHKKALYEALEKVKAELMLLGFISLLLVVLQDPVSQICIPRKVASTWHPCTHHQEMVKYGKDYVDDGRKILEDFDSTDVFGPRRSLATKGYDKCAEKGKVALVSAYGIHQLHIFIFVLAVFHILYCIITYALGKTKMKKWKSWEKETKTIEYQYANDPERFRFARDTSFGRRHLNMWSKSSVTLWITCFFRQFFGSVTKVDYLTLRHGFIMAHLPAGSAARFDFQKYIQRSLEEDFTVVVGISPLIWCIAVLFILTNTNGWASYLWLPFIPLLVILVVGAKLQMIISKLGLRIQDKGDVVKGAPVVEPGDDLFWFGRPRLILFLIHLVLFTNAFQLAFFVWSTYEFTLRNCFHHKNEDIAIRITMGVLIQVICSYITLPLYALVTQMGTSMRPTIFNDRVANALKKWHHTAKKQTKHGQSGSNTPHSSRPATPTHGMSPVHLLHNYHNRSSLDQQTSFTASPSPPRFSDYSGHGHGHHQFFDPESQNHSYQREITDSEFSNSHHPHTDVASPVREEMQIVEHVKVDLPEFTFKK

核苷酸序列：

ATGGCGATAAAAGAGCGAACACTGGAGGAGACACCAACATGGGCTGTTGCTGTTGTTTGCTTCGTTCTCCTTTTCATTTCCATCATGATCGAATATTTCTTGCACTTTGTTGGTCACTGGTTTAAAAAGAAGCACAAGAAAGCTTTATATGAAGCTCTTGAAAAGGTTAAAGCAGAATTGATGCTACTGGGATTCATATCGCTTCTATTGGTGGTATTGCAAGATCCAGTCTCTCAGATTTGCATTCCAAGAAAAGTTGCTTCGACTTGGCATCCTTGTACCCACCATCAAGAAATGGTTAAATACGGTAAAGATTATGTCGACGATGGCCGCAAGATTCTTGAAGACTTTGACTCCACCGACGTTTTTGGTCCTCGCCGCAGTTTAGCCACCAAAGGTTATGACAAATGCGCAGAAAAGGGAAAAGTAGCATTAGTATCTGCATATGGTATCCACCAGCTGCATATATTCATCTTTGTGCTTGCTGTTTTTCATATCCTCTACTGCATTATAACCTATGCTTTGGGGAAGACCAAGATGAAGAAATGGAAGTCATGGGAGAAAGAGACCAAAACAATTGAGTACCAATATGCCAATGATCCCGAGAGGTTCAGATTTGCAAGAGACACATCGTTCGGACGTAGACATTTGAACATGTGGAGCAAGTCTTCCGTTACCCTCTGGATTACATGTTTCTTCAGACAATTCTTTGGATCAGTGACAAAAGTGGATTATCTTACACTAAGACATGGCTTTATTATGGCGCATTTGCCAGCAGGAAGTGCAGCTCGTTTCGATTTCCAAAAATACATTCAAAGATCTTTGGAAGAAGATTTCACGGTGGTGGTCGGCATAAGTCCATTGATATGGTGCATTGCTGTCTTATTCATATTGACCAACACAAATGGATGGGCTTCATATCTTTGGTTGCCCTTCATCCCATTGCTTGTGATATTGGTAGTAGGAGCAAAACTTCAAATGATAATATCCAAGTTAGGATTAAGGATTCAAGACAAAGGAGATGTGGTTAAAGGAGCTCCTGTGGTTGAACCGGGTGATGATCTCTTCTGGTTTGGTCGTCCTCGTTTGATCCTCTTCCTCATCCACTTGGTCCTTTTCACGAATGCATTTCAACTGGCTTTCTTCGTTTGGAGCACTTACGAATTCACACTCCGAAACTGCTTCCACCACAAAAACGAAGATATTGCAATTAGGATCACCATGGGGGTATTAATACAAGTTATATGCAGCTACATCACTCTACCTCTCTATGCTCTTGTTACTCAGATGGGAACTTCAATGAGGCCGACCATATTCAACGATAGGGTAGCAAATGCATTGAAGAAATGGCACCACACAGCCAAGAAACAGACGAAACATGGACAGTCGGGATCTAACACACCTCACTCTAGCCGCCCTGCTACGCCAACTCATGGCATGTCACCGGTGCATCTCCTCCACAACTACCATAACCGCAGCAGCCTCGACCAACAAACCAGCTTCACGGCCTCTCCTTCTCCTCCTAGATTCTCTGATTATAGCGGCCATGGCCATGGCCATCATCAGTTCTTCGACCCTGAATCTCAGAATCACTCTTACCAGCGTGAGATCACAGATTCTGAATTCAGCAACAGTCATCATCCCCATACTGACGTTGCAAGTCCTGTTAGAGAAGAGATGCAGATTGTTGAGCATGTCAAGGTTGATTTGCCTGAATTTACCTTCAAGAAGTAG

carubv10009086m.g

氨基酸序列：

MADATLEETSTWTVAVVCFVLLLISIALEHLLHTLGTWFKKKHKSDLFEALEKVKAELMLLGFISLLLTIAQAPISHICISKKLASIMQPCSPAQAAKLKADRAKKEGGYGEKPGGKLLLELAEFYIPRRSLAGKVENTCEKKGKVPFVSYYGIHQLHIFIFVLAVVHIIYCIVIYALGKTKMRRWQPWEEEAKSIEYQFSNDPERFRFARDTSFGRRHLSFWSKRSVTLWTVCFFRQFFGSITKVDYLTLRHGFITAHFAPGQSSYDFRKLIQRTLEKDFKTVVEISPVIWFVAVLFLLTNTSGLYSHFWLPFIPLVVSLIVGTKLEVIITQLGQSIQEKDEIVRGAPVVRPKDDLFWFSKPRFVLFLIHLVLFTNAFQLAFFAWSTFSFGIKNCFHASIPDVVTRLVSFCTDTLQLCDSSTLCTCYSHGYSNEANSIQRRSSHSIREVASDGEEPD

核苷酸序列：

ATGGCAGACGCGACTTTAGAGGAGACCTCTACGTGGACAGTAGCTGTGGTTTGCTTCGTCTTACTACTCATTTCGATTGCGCTCGAACATTTGCTTCACACACTTGGAACCTGGTTTAAAAAGAAGCACAAAAGCGATCTATTTGAAGCTCTTGAAAAGGTCAAAGCAGAGCTTATGTTGTTGGGATTCATATCACTACTCCTAACAATTGCACAAGCACCAATCTCACATATTTGCATCTCCAAGAAACTTGCATCGATAATGCAACCTTGCAGTCCCGCCCAAGCAGCGAAATTAAAGGCCGATAGGGCAAAGAAAGAAGGTGGATATGGAGAAAAACCCGGTGGAAAACTTCTTCTTGAGCTGGCTGAATTTTATATCCCTAGAAGAAGTTTAGCCGGCAAAGTTGAGAACACATGCGAAAAGAAGGGGAAAGTGCCTTTTGTATCTTATTATGGAATCCACCAGCTGCATATATTCATCTTCGTGCTCGCAGTGGTTCATATTATTTACTGCATTGTTATTTATGCTCTCGGAAAGACCAAGATGAGAAGGTGGCAGCCGTGGGAGGAGGAGGCAAAGTCAATAGAGTATCAGTTTTCCAACGATCCTGAGAGGTTCAGGTTTGCGAGGGACACATCTTTTGGGAGAAGACATCTCAGTTTCTGGAGCAAGAGGAGTGTTACACTATGGACTGTGTGTTTTTTCAGACAGTTCTTTGGATCCATCACCAAAGTTGATTACTTAACATTAAGACATGGTTTTATCACGGCGCATTTCGCTCCCGGCCAATCAAGCTACGATTTCCGTAAGTTAATCCAGAGAACGTTAGAGAAAGACTTCAAAACCGTTGTTGAAATCAGTCCTGTTATCTGGTTTGTCGCCGTGCTATTCCTCTTGACCAATACAAGTGGGTTATATTCTCACTTCTGGTTACCATTCATTCCACTAGTCGTAAGTCTAATCGTTGGAACAAAGCTTGAAGTCATAATAACACAATTGGGTCAAAGTATCCAAGAGAAAGACGAAATAGTGAGAGGCGCCCCTGTGGTTCGGCCTAAAGATGACCTCTTCTGGTTTAGCAAGCCACGTTTCGTTCTTTTCCTTATCCACTTGGTCCTCTTTACGAATGCATTTCAACTTGCCTTCTTTGCCTGGAGCACGTTTTCATTCGGGATCAAGAATTGTTTCCATGCAAGCATTCCAGATGTGGTCACTAGACTTGTTAGCTTTTGTACAGATACTTTGCAGCTATGTGACTCTTCCACTCTATGCACTTGTTACTCACATGGGTACTCAAATGAAGCCAACAGTATTCAACGAAGGAGTAGCCACAGCATTAGAGAAGTGGCATCAGACGGCGAAGAACCAGACTAA

carubv10008691m.g

氨基酸序列：

MANQVKERTLEETSTWAVAVVCFVLLFISIVLEHSIHKIGTWFRKKHKKALFEALEKVKAELMLLGFISLLLTIGQTPISNICISEQVASTMHPCSAAEEARKYHKKDPAKKEHGDGDGEKSGRRLLLELAESYIPRRSLATKGYDKCAEKGKVAFVSAYGIHQLHIFIFVLAVVHVIYCIVTYALGKTKMRRWKSWEEETKTIEYQYSNDPERFRFARDTSFGRRHLNFWSKSSVTLWVVCFFRQFFGSVTKVDYLALRHGFIMAHLAHGSESRFDFRKYIQRSLEEDFKTVVEISPVIWF?VAVLFLLTNTNGLRSYLWLPFIPLIVILIVGTKLQVIITKLGLRIQEKGDVVRGAPVVQPGDDLFWFGKPRFILFLIHLVLFTNAFQLAFFAWSTYEFNLRNCFHESNADVVIRIAVGVVVQILCSYVTLPLYALVTQMGTKMKPTVFNERVATALKKWHHTAKSQTKHGRHSESNTPFSSRPTTPTHGSSPIHLLHNFNNRNVDSYPTSPSPRYSGHGHHDEHQFWDPESQRQEAETSTHHSLAHESSERKPALASVELPPIRTSKSLKDFSFKR

核苷酸序列：

ATGGCGAATCAAGTTAAGGAGCGGACTTTAGAAGAGACCTCTACGTGGGCAGTAGCTGTGGTTTGCTTCGTCTTACTATTTATTTCGATTGTTCTCGAACATTCGATTCACAAAATTGGAACCTGGTTTAGAAAGAAGCACAAGAAGGCTCTATTTGAAGCTCTTGAAAAGGTCAAAGCAGAGCTTATGCTGTTGGGATTCATATCACTACTCCTCACTATTGGACAAACGCCAATCTCAAATATCTGCATCTCCGAGCAGGTTGCATCGACAATGCACCCTTGCAGCGCCGCGGAAGAAGCTAGAAAATACCACAAGAAAGACCCCGCAAAGAAAGAACATGGAGATGGAGATGGAGAAAAATCGGGTCGAAGACTTCTTCTTGAGTTAGCTGAATCTTATATCCCTAGACGAAGTTTAGCCACCAAAGGTTATGACAAATGTGCAGAGAAGGGGAAAGTGGCTTTTGTATCTGCTTATGGAATCCACCAGTTGCATATATTCATCTTTGTGCTGGCCGTGGTTCATGTTATTTATTGCATTGTTACTTATGCTCTCGGAAAGACTAAGATGAGGAGGTGGAAGTCATGGGAGGAGGAGACGAAGACAATAGAGTATCAGTATTCCAATGATCCTGAGAGGTTCAGGTTTGCGAGGGACACATCCTTTGGGAGAAGACATCTCAATTTCTGGAGTAAGTCGAGTGTTACACTATGGGTTGTGTGTTTTTTCAGACAGTTCTTTGGATCTGTCACCAAAGTTGATTACTTAGCACTAAGACATGGTTTCATCATGGCGCATTTGGCTCACGGGAGCGAATCAAGATTTGATTTCCGCAAGTATATTCAGAGATCATTAGAGGAAGACTTCAAAACTGTTGTTGAAATCAGTCCGGTTATTTGGTTTGTCGCGGTGCTATTCCTCTTGACCAATACAAATGGACTACGTTCTTACCTTTGGTTACCATTTATTCCACTAATCGTAATTCTAATAGTTGGAACAAAGCTTCAAGTCATAATTACCAAATTGGGTCTAAGAATCCAAGAGAAAGGTGATGTGGTGAGAGGTGCTCCGGTGGTTCAGCCTGGTGATGACCTCTTCTGGTTTGGCAAGCCACGGTTCATTCTTTTTCTTATCCACTTGGTCCTCTTTACGAATGCATTTCAACTAGCCTTCTTTGCCTGGAGTACGTATGAGTTCAATCTCAGGAATTGTTTCCATGAAAGCAATGCAGATGTGGTCATTAGGATTGCAGTTGGAGTTGTTGTGCAGATACTTTGCAGCTATGTGACTCTTCCACTCTATGCACTTGTTACTCAGATGGGTACTAAGATGAAGCCAACAGTATTTAACGAAAGAGTAGCCACAGCATTAAAGAAGTGGCATCACACGGCAAAGAGCCAGACTAAACACGGAAGACACTCTGAATCAAACACACCTTTCTCTAGCCGTCCAACCACACCAACACATGGCTCATCTCCCATCCATCTCCTTCACAATTTCAATAACCGGAACGTTGACAGTTACCCAACTTCTCCTTCTCCTAGATACTCTGGTCATGGTCATCATGATGAACACCAGTTTTGGGATCCTGAGTCTCAACGCCAAGAAGCTGAAACGTCCACACATCATTCTCTTGCGCATGAAAGCTCAGAAAGGAAGCCGGCTCTTGCATCTGTTGAACTTCCTCCAATAAGGACTAGCAAAAGCTTAAAAGATTTCTCTTTTAAGAGATGA

carubv10020030m.g

氨基酸序列：

MAEQVKEKTLEETSTWAVAVVCFVLLLISIVIENLIHKIGSWFKNKNKKALYEALEKVKAELMLMGFISLLLTIGQGYISNICIPKNIAASMHPCSAAEEARKYGKKLVPKVQEDGGDDDDNSRRKLLQLVDSIIPRRSLATKGYDKCAEKGKVAFVSAYGMHQLHIFIFVLAVCHVIYCIVTYALGKTKMRRWKRWEQETKTIEYQYSHDPERFRFARDTSFGRRHLNFWSRTTVTLWIVCFFRQFFRSVTKVDYLTLRHGFIMAHLAPGSDARFDFRKYIQRSLEEDFKTVVEINPVIWFVAVLFLLTNTNGLNSYLWLPFIPFVVILIVGTKLQVIITKLGLRIQEKGDVVKGTPLVQPSDHFFWFGRPRFILFLIHLVLFTNAFQLAFFVWSTYEFGLKNCFHESSVDVIIRISIGLIVQILCSYVTLPLYALVTQMGSKMKPTVFNERVAIALKSWHHTAKKQIKHGRTSESTTPFSSRPTTPTHGSSPIHLLRNVPHKRSRSVDDNSFANSMSPRRNSDFDSWDPESQHETAETSTNSNHRSRFREEESEKKLGPSSSVELPPGPGQIRTQHDISNISLRDFSFKR

核苷酸序列：

ATGGCGGAACAAGTGAAAGAAAAAACATTAGAGGAAACTTCTACATGGGCCGTCGCTGTGGTTTGCTTCGTCTTGCTTCTGATTTCTATTGTTATTGAGAATCTTATTCATAAAATTGGATCCTGGTTTAAAAACAAGAACAAAAAAGCTTTATATGAAGCTCTTGAAAAAGTGAAAGCAGAGCTTATGCTGATGGGATTCATATCACTACTGCTTACTATTGGACAAGGCTACATCTCGAATATTTGCATCCCTAAGAACATCGCAGCGTCGATGCACCCTTGTAGTGCAGCCGAAGAAGCGAGAAAGTACGGTAAGAAATTAGTCCCAAAGGTCCAGGAAGACGGAGGAGACGACGATGACAACTCGCGTCGAAAGCTTCTCCAATTAGTCGATTCTATCATTCCTCGAAGGAGTTTGGCTACCAAAGGTTATGATAAGTGTGCAGAGAAGGGGAAAGTCGCTTTTGTATCGGCTTATGGCATGCATCAGCTGCATATATTCATCTTTGTTCTTGCGGTTTGTCATGTGATTTACTGCATTGTTACTTATGCTTTGGGGAAGACCAAGATGAGAAGGTGGAAGAGGTGGGAACAGGAGACGAAGACAATCGAATATCAGTATTCACACGATCCCGAGAGGTTTAGGTTCGCGAGGGATACATCTTTCGGGCGTAGACATCTGAATTTCTGGAGCAGAACAACTGTTACGCTGTGGATTGTATGTTTCTTCAGACAGTTCTTTAGATCAGTAACCAAAGTTGATTACTTAACACTGAGACATGGTTTCATCATGGCTCATTTGGCTCCTGGGAGCGATGCAAGATTCGATTTCCGAAAGTACATTCAGAGATCATTAGAGGAAGACTTTAAAACCGTTGTCGAGATCAATCCTGTGATCTGGTTCGTCGCTGTCTTGTTTCTCCTGACCAACACAAACGGATTGAATTCTTACCTCTGGTTACCTTTCATTCCTTTTGTCGTGATTCTTATCGTTGGGACAAAACTTCAAGTGATAATAACAAAACTAGGACTACGAATCCAAGAGAAAGGCGACGTAGTAAAAGGCACACCGCTTGTTCAGCCCAGTGATCATTTCTTCTGGTTCGGTCGCCCACGTTTCATTCTCTTCCTCATTCACTTGGTTCTCTTCACGAACGCGTTTCAACTCGCTTTCTTTGTCTGGAGTACGTATGAATTCGGTCTAAAGAACTGTTTCCATGAAAGCAGTGTAGATGTAATCATCAGAATCTCAATCGGACTTATTGTCCAGATTCTTTGCAGCTACGTTACTCTTCCTCTTTACGCTCTTAGTCACTCAGATGGGTTCAAAGATGAAACCAACAGTGTTCAACGAGAGAGTAGCAATAGCATTAAAGAGTTGGCACCACACAGCAAAGAAACAGATCAAACATGGAAGAACCTCAGAATCAACCACACCTTTCTCTAGCCGACCAACAACACCAACACATGGTTCTTCTCCGATTCATCTCCTCCGCAACGTCCCTCACAAACGAAGCAGAAGCGTTGACGATAACAGCTTTGCGAATTCAATGTCTCCGAGAAGAAACTCTGATTTCGATTCGTGGGATCCTGAGTCTCAACACGAAACTGCTGAGACTTCGACGAACTCAAATCATCGTTCAAGGTTTAGAGAAGAAGAATCGGAGAAAAAGCTTGGACCTTCTTCATCTGTTGAACTTCCTCCTGGACCTGGTCAGATACGAACTCAGCATGATATTAGTAATATCAGCTTAAGGGATTTTTCGTTTAAGCGGTGA。

2.權利要求1所述快速鑒定薺菜白粉病基因的應用，包括：

1)攜帶有抗白粉病MLO基因的育種材料的創制或者新品種創制：利用權利1所給出的MLO基因源為親本材料，在雜交、回交后代中，可以獲得轉育有MLO抗性基因的育種材料。

2)抗白粉病基礎理論研究：對權利1的MLO型抗白粉病基因進行分子標記分析；對該基因進行分子作圖或者基因定位；對該基因進行克隆以及基因間互作分析。

3)抗白粉病轉基因研究：利用權利要求1所給出的MLO型白粉病基因進行轉基因研究。